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FROM DAY 1
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+-SH safe2280919 +-SH artist:brenda hickey1195 +-SH idw22571 +-SH smarty pants1603 +-SH spike95503 +-SH twilight sparkle371591 +-SH pony1710967 +-SH friendship is magic #40166 +-SH g42123149 +-SH my little pony: friendship is magic (idw)6677 +-SH spoiler:comic13647 +-SH baby spike800 +-SH crib811 +-SH crying58289 +-SH cute280293 +-SH female1911779 +-SH filly105864 +-SH filly twilight sparkle3649 +-SH heart84104 +-SH twiabetes16314 +-SH younger24537
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In-universe I guess the worst you could say is she’d rather confide in an inanimate doll than the baby, which isn’t saying much since neither can respond.
But on a meta level it’s because the first two panels build up the expectation that Twilight is confiding in Spike, only to yank that away in the last; Spike’s expression reflects this.