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Description

I would post more instances where other members of the mane six got tied up or gagged, but there was too many and I think you get the idea.

safe2272826 edit181033 edited screencap95728 screencap302707 angel bunny11739 applejack208349 fluttershy269643 pinkie pie266381 rainbow dash291451 rarity226221 twilight sparkle371991 earth pony548065 pegasus539154 pony1705830 rabbit9819 unicorn584185 daring done?1066 dragon quest1113 g42131364 my little pony: friendship is magic267959 putting your hoof down986 secret of my excess1000 secrets and pies907 animal9131 blindfold5996 bondage49345 female1914967 high res413247 mare809838 rope16915 tied up9448
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Background Pony #40DF
What about in “Boast Busters” when Trixie hogtied Applejack and gagged her with an apple?
 
Or in “Fall Weather Friends” when Rainbow Dash tied herself up?
Background Pony #DD05
@DragonRanger  
If Equestria girls count, Fluttershy got lassoed by Applejack and tied to Rainbow Dash in tickest.
 
And if we’re counting gags, Pinkie Pie got her mouth removed.
Background Pony #239D
@DragonRanger Pinkie pie got tied to train tracks in Mystery on the Friendship Express.
DragonRanger
Lunar Supporter - Helped forge New Lunar Republic's freedom in the face of the Solar Empire's oppressive tyrannical regime (April Fools 2023).
Non-Fungible Trixie -
My Little Pony - 1992 Edition
Wallet After Summer Sale -
Fine Arts - Two hundred uploads with a score of over a hundred (Safe/Suggestive)
Perfect Pony Plot Provider - 10+ uploads with over 350 upvotes or more (Questionable/Explicit)

In order of who gets tied up the most, I want to say it’s Rainbow Dash at the top. This pic shows off a lot of those moments.
 
Then there’s Applejack and Twilight, who are probably about even.
 
Pinkie and Fluttershy I can’t really think of any outside of the ones shown above.